Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
XianPing Fang, and SongLin Ruan
Posters: J Proteomics Bioinform
The strawberry (Fragaria ananassa) can reproduce asexually through stolons, including the proximal internode (I-1) and the distal internode (I-2), which has a strong tendency to form adventitious roots at the nodes. Understanding the different development mechanisms of I-1 and I-2 will facilitate nursery plant production. Here we report comparative internode proteomics of strawberry stolons. Proteins from I-1 and I-2 samples were extracted and separated by two-dimensional gel electrophoresis. A total of 164 protein spots from I-1 gels and 200 protein spots from I-2 gels were selected carefully. Out of these spots, a total of 38 and 52 proteins were identified by mass spectrometry in I-1 and I-2, respectively. All the identified proteins were categorized into 8 and 10 groups according to their cellular compartmentalization and biological functionality, respectively. Enzymes necessary for carbohydrate metabolism and photosynthesis dominated in the set of identified proteins. Analysis of protein expression patterns revealed that the ubiquitin-proteasome pathway and sugar-hormone connections may play important roles in the growth and development of I-2. Furthermore, protein-protein interactions studies revealed many proteins were involved in I-2 development mechanism as a dynamic network. Finally, based on our proteomic results, we brought forward a possible schematic representation of mechanism associated with the adventitious root formation of new clonal plants in I-2.