Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Moon-Chang Baek, Chan-Hyeong Lee, Eun-Ju Im, Pyong-Gon Moon, Jong-Sup Bae, Sang-Hyun Kim and Taeg Kyu Kwon
Kyungpook National University�Daegu, South Korea
Keimyung University�Daegu, South Korea
Posters & Accepted Abstracts: J Chromatogr Sep Tech
Exosomes are informative vesicles containing unique information about the condition of cells. Therefore, many researchers have suggested that exosomal proteins can be potential biomarkers for diseases such as cancer. Colon cancer is one of the most common forms of cancer related death in the world. Despite current biomarkers such as carcinoembryonic antigen used in hospitals, demand for novel biomarkers for colon cancer diagnosis is increasing because of their low sensitivity. This study was focused on identification of biomarkers for diagnosis of colon cancer through proteomic approaches to investigating exosomes from HT-29 and HCT-116 colon cancer cell lines, which were isolated by differential ultracentrifugation. These exosomes were characterized by western blot analysis, nanoparticle tracking analysis, and transmission electron microscopy. Exosomal proteins were analyzed using LC-MS/MS. Five selected proteins were shown to be upregulated in colon cancer by western blot analysis. In addition, tetraspanin-1 among the candidate proteins was upregulated in exosomes from colon cancer patients compared with healthy controls. These results suggest that tetraspanin-1 is a potential diagnostic biomarker. Additionally, the experimental strategy described in this study is expected to help identify cancer-specific and body fluidaccessible biomarkers.