Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Jianjun Liu, Xiaohu Ren, Rongxia Deng, Jiawen Ruan, Jiacheng Zhong and Wei Liu
Jianjun Liu, Xiaohu Ren, Rongxia Deng, Jiawen Ruan, Jiacheng Zhong and Wei Liu
Scientific Tracks Abstracts: J Chromatogr Sep Tech
Trichloroethylene (TCE) was widely used in industrial productions and turned into an environmental and occupational toxicant. Expression of SET protein was previously found as dose-dependent with TCE in human liver cells. It is also an important inhibitor of phosphatase 2A and histone acetyltransferase. However SET-mediated abnormal histone modification and protein phosphorylation in TCE induced hepatic cytotoxicity remain poorly understood. SET-mediated protein phosphorylation in TCE-induced hepatic cytotoxicity was analyzed by iTRAQ labeling and IMAC enrichment based quantification proteomics study. 14 phosphopeptides from 13 proteins were found as SET-mediated (de)-phosphorylation in hepatic cytotoxicity of TCE. Furthermore, nucleolin was found self-regulating by SET-mediated phosphorylation through enhanced interaction with c-myc and inhibiting of c-myc. SET-mediated histone methylation and acetylation were analyzed by TAU-SDS-PAGE seperation combining with LC-ESI-MS based label-free quantification. The ubiquitinated and sumoylated histones were first enriched by specific antibodies then labeled with stable isotopic dimethylation reagents and analyzed by LC- ESI-MS. 12 acetylated peptides, 11 methylated peptides, 10 ubiquinated peptides and 6 sumoylation peptides were found as SET-mediated alteration in hepatic cytotoxicity of TCE. Our findings provided molecular-level evidence further supporting our previous findings that knockdown of SET attenuated TCE-induced hepatic cytotoxicity. SET-mediated self-regulating of nucleolin and abmornal histone modifications further revealed the molecular mechanism of SET-mediated hepatic cytotoxicity of TCE.
Jianjun Liu has been studying the mechanisms of TCE-induced hepatotoxicity for several years. She is a doctoral supervisor. She is the director of Key Laboratory of Modern Toxicology of Shenzhen, a division of Shenzhen Center for Disease Control and Prevention. She has published more than 60 papers in international and chinese journals and has authorized 3 patents of invention.
Email: junii8@126.com