Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Igor Sokolov
Tufts University, USA
Scientific Tracks Abstracts: J Proteomics Bioinform
Mechanics of eukaryotic cells at the nanoscale is a challenging problem due to complexity of cells. Besides fundamental interest, such measurements are important because it has been demonstrated that the elastic modulus of cells can correlate with various diseases and even aging. Atomic force microscopy (AFM) is the technique of use. At the same time, there is a lot of confusion in the process of measurements of the elastic modulus of cells. In the talk, author will present the modern overview of the topic, describe step-by-step how to do the measurements of the elastic modulus in quantitative manner that is independent of the particular microscope, AFM probe, and mechanical model. Recent Publications 1. Dokukin M E and Sokolov I (2017) Nanoscale compositional mapping of cells, tissues, and polymers with ringing mode of atomic force microscopy. Scientific reports 7(1):11828. 2. Dokukin M E, Kuroki H, Minko S and Sokolov I (2017) AFM Study of Polymer Brush Grafted to Deformable Surfaces: Quantitative Properties of the Brush and Substrate Mechanics. Macromolecules 50(1):275â??282. 3. Simon M, Dokukin M, Kalaparthi V, Spedden E, Sokolov I and Staii C (2016) Load rate and temperature dependent mechanical properties of the cortical neuron and its pericellular layer measured by atomic force microscopy. Langmuir 32(4):1111-1119. 4. Guz N V, Patel S J, Dokukin M E, Clarkson B and Sokolov I (2016) Biophysical differences between chronic myelogenous leukemia quiescent and proliferating stem/progenitor cells. Nanomedicine 12(8):2429-2437. 5. Dokukin M and Sokolov I (2015) High-resolution high-speed dynamic mechanical spectroscopy of cells and other soft materials with the help of atomic force microscopy. Scientific reports 5:12630.