Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Ahmed Aldarmahi
King Saud bin Abdulaziz University for Health Sciences, Saudi Arabia
Posters & Accepted Abstracts: J Proteomics Bioinform
Proteomics is very much a technology-driven field. The ambition is to identify, quantify and to assess the state of post-translational modification and interaction partners for every protein in the cell. Analysis of the proteome is exacerbated by the huge dynamic concentration range of proteins in the cellular environment. The impact of high throughput such as mass spectrometry-based proteomics in the field of reproductive medicine has been made in recent years. Much effort has been applied to develop and improve the preparation techniques for proteomic samples to be able to detect the low abundant proteins. One partial remedy is to deplete the proteomic samples. We compared two techniques for the cleaning and purification of oviductal and uterine horn secretory proteomes in primary cell culture system. We found that no significant difference was observed in number of spots detected between the samples prepared by acetone precipitation technique to those prepared by commercial kit. Protein samples were run through strong cation exchange (SCX) liquid chromatography in order to fractionate samples of major proteins. Protein identification by mass spectrometry revealed a significant detection of low abundant proteins in comparison to high abundant proteins. In silico model of interaction of gametes with female reproductive tract has been established and analyzed. Our findings showed that the oviduct distinguishes between different non-self-entities by yielding specific response to each entity. Future experiments should be directed toward understanding the sensory mechanism used by oviduct to distinguish spermatozoa from other entities within the female reproductive tract.
Email: aldarmahiah@ngha.med.sa