Journal of Chromatography & Separation Techniques
Open Access

Results on the determination of fatty acids in biological samples by applying gas chromatography

World Congress on Chromatography

September 21-23, 2016 Amsterdam, Netherlands

Ropota Mariana and Olteanu Margareta

IBNA Balotesti, Romania

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Analytical method validation is the confirmation by examination and provision of objective evidence that certain specific requirements for intentional application are achieved. So validation of analytical quality assurance represents the first step in a laboratory. The fatty acids were determined by gas chromatography which involves the transformation of the fatty acids from the sample in methyl esters and separation of the components in the chromatographic column, their identification by comparison with the standard chromatograms. The method complies with standard SR CEN ISO/TS 17764 -2: 2008, used a Perkin Elmer-Clarus 500 chromatograph with capillary injection, high polarity stationary phase (BPX70: 60 mx0.25 mm inner diameter and 0.25 �?¼m film thickness). The method was validated â�?�?in houseâ�?, and used as methylated fatty acids standard solution Mix 37 Component FAME; 10 mg/mL, (CRM) soybean oil. We determined the following parameters: accuracy=98.72%, coefficient of variation of repeatability RSD=0.414%, detection limit LoD=0.002349 �?¼g/mL, quantification limit LoQ=0.05683 �?¼g/mL and recovery R=98.84%, according to SR EN ISO/CEI 17025: 2005, all values being within the admitted range: RSD: 80â�?�?120%, LoQ>LoD and 80120%. The following concentrations of fatty acids were determined in samples of eggs, expressed per 100 g fat extracted from the yolk. Thus, �?±-linolenic acid has ranged between: 0.22�?±0.3 g (C) and 1.19�?±0.13 g/100 g fat, total omega-3 has values between: 1.37�?±0.09 g (C) 4.84�?±0.32 g (E1) and total omega-6 has values between: 24.61�?±1.38 g (C) and 20.91�?±1.08 g/100 g fat (E1).

Biography :

Email: m.ropota@yahoo.com