Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Amr Amin, Alaaeldin A. Hamza, Khuloud Bajbouj, S. Salman Ashraf and Sayel Daoud
Accepted Abstracts: J Cell Sci Ther
Saff ron has been proposed as a promising candidate for cancer chemoprevention. Th e purpose of this investigation was to investigate the chemopreventive action and the possible mechanisms of saff ron against diethylnitrosamine (DEN)-induced liver cancer in rats. Administration of saff ron (75 mg/kg per day) started two weeks prior to the DEN injection and was continued for 22 weeks. Saff ron signifi cantly reduced the DEN-induced increase in the number and the incidence of hepatic nodules. Saff ron also decreased the number and the area of placental glutathione-S-transferase positive foci in livers of DEN-treated rats. Furthermore, saff ron counteracted DEN-induced oxidative stress in rats as assessed by restoration of superoxide dismutase, catalase, and glutathione-S-transferase levels and diminishing of myeloperoxidase activity, malondialdehyde and protein carbonyl formation in liver. Immunehistochemistry showed that saff ron inhibited the DEN mediated elevations in numbers of cells positive for Ki-67, cyclooxygenase 2, inducible nitric oxide synthase, nuclear factor-kappa Bp-65 and the phosphorylated tumor necrosis factor receptor. Saff ron also blocked the depletion in the number of cells positive for TUNEL and M30 CytoDeath in liver tissues of DEN-treated rats. In vitro experiments carried out using HepG2 cells confi rmed those fi ndings and showed inhibition of NFkB activation, increased cleavage of caspase-3, and DNA damage and cell cycle arrest upon saff ron treatment. Th e present study provides evidence that saff ron exerts a signifi cant chemopreventive eff ect against liver cancer through inhibition of cell proliferation and induction of apoptosis. Th is report also shows some evidence that saff ron protects rat liver from cancer via modulating oxidative damage and suppressing infl ammatory response
Prof. Amin is a graduate faculty at UAE University who supervised many graduate theses. He earned his PhD from University of Illinois at Chicago and received a postdoctoral training at University of Pennsylvania School of Medicine. After joining UAEU Prof. Amin?s focus was redirected to the fi eld of preventive medicine. His lab is interested in natural product?s protection against diabetes and cancer. He has published many articles, reviews and book chapters in reputable journals. He serves on the editorial boards and as a reviewer of many international journals. Prof. Amin is also the recipient of many national and international awards