Drug Designing: Open Access
Open Access
ISSN: 2169-0138
ISSN: 2169-0138
Zerihun Tesfaye Nigussie
Addis Ababa University, Ethiopia
Scientific Tracks Abstracts: Drug Des
Objective: To evaluate antileishmanial activity of methanolic extract of Aloe otallensis (A. otallensis) on the promastigote stage of Leishmania donovani (L. donovani) as compared to standard drugs and to screen its phytochemical constituents. Methods: Phytochemical screening was done by using the method mentioned by Evans and Trease on methanolic extract of the exudates of Aloe otallensis leaves. The extract was also evaluated for in vitro antileishmanial activity against L. donavani which is found from the Parasitology Unit of Black Lion Hospital. The result was compared to standard drugs of sodium stibogluconate, milfostin and paramomycin. Results: The extract has a good antileishmanial activity with an IC50 of 0.123 0 μg/mL on L. donovani (AM 563). The experimental data showed that relatively it had better activity than paramomycin and milfostin but less activity than sodium stibogluconate. The data analyses were done by GraphPad Prism version 5 software after it was read by ELISA reader at the wave length of 650 nm. The phytochemical screening of the exudates of A. otallensis showed the presence of phenol, alkaloid and saponin. Conclusion: The methanol extract of the exudates of A.otallensis has a good anti-leishmaniasis activity this may be attributed to phenol, alkaloid and saponin present in the plant. But it needs further analysis for the conformation of which constituent presents in high concentration to know which one has the strongest effect. Keywords: Anti-leishmaniasis, Aloe otallensis, Leishmania donovani, IC50.
Zerihun Tesfaye Nigussie, graduated in Chemistry Diploma from Kotebe teaching college on JUN, 2006 and Bachelor of pharmacy on July 2011 from Addis Ababa University. He is employed at Addis Ababa University aklilu Lema institution of path biology Research center on September 2007 as Technical Assistance and served for the past 5 years. He is Giving Technical support for Master and PhD students both on the field and Laboratory. Currently, He is working as a chief Pharmacist at Addis Ababa University, college of Health Science, Black lion specialized Teaching Hospital in Mentoring under graduate pharmacy students who are coming to the hospital for clinical attachment both at the ward and dispensary area. He is also participating in some of Clinical research which is under go in the Hospital beside the routine work.