Journal of Chromatography & Separation Techniques
Open Access

Simultaneous determination of sofosbuvir, paracetamol and methionine in rat plasma using thin-layer chromatography and its application to pharmacokinetic study

4^th World Congress on Chromatography

August 07-09, 2017 | Rome, Italy

Nada Abdelwahab

Beni-Suef University, Egypt

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Sofosbuvir (SOF) is a widely used drug for treatment of chronic hepatitis C while paracetamol (PAR) is the recommended analgesic for patients with hepatitis C because of its effectiveness and safety. Combination of PAR with methionine (MET) is preferred to reduce the severity of liver damage that may be produced from PAR overdose. A sensitive and highly selective TLC-densitometric method was developed for the first time for simultaneous analysis of SOF and the accompanied medications PAR and MET in the presence of the internal standard, naphazoline HCl (NAP) in rat plasma. Complete separation between the studied components peaks and plasma peak was obtained where Rf value of MET=0.18, NAP=0.39, PAR=0.59 and SOF=0.82. FDA recommendations for bioanalytical method validation were obeyed. The linearity of the method was assessed over the concentrations range 160-3000 ng mL-1 for both SOF and PAR and 300-3000 ng mL-1 for MET. Moreover, the accuracy, intra-and inter-day precision of the quality control samples at low, medium and high concentration levels exhibited relative standard deviations (RSD)<10%. Freezing-thawing stability was also tested; additionally pharmacokinetic and pharmacodynamics co-relation of the studied drugs in animal model has been done. The developed method can be easily used during accurate monitoring of the studied drugs.

Biography :

Email: nadasayed2003@yahoo.com