Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Nethravathi P, Suresh Kumar Raman, Babu B, Karthik Yamjala and Narenderan S T
JSS College of Pharmacy, India
JSS Academy of Higher Education and Research (Deemed to be University), India
Posters & Accepted Abstracts: J Chromatogr Sep Tech
Objective: A novel, accurate, precise and rapid LC-MS/MS method has been developed for the simultaneous
estimation of artesunate (ART), dihydroartemisinin (DHA) and quercetin (QRT) in rat plasma.
Methods: Agilent zorbax C18 column (3 μm, 50 mm�4.6 mm) was used a stationary phase and mobile
phase constitutes 0.1% formic acid (90 volumes) and methanol (10 volumes), at a flow rate of 0.4mLâ?¢minâ??1
and injection volume of 10 μL. Artemisinin (ART) was used as internal standard. For collision induced
dissociation experiments argon gas was employed. Mass detection was performed by electrospray ionization
tandem mass spectrometry via multiple reaction monitoring (MRM) in positive mode except for quercetin
where negative ionization was used. Plasma samples were subjected to solid phase extraction using methanol
as eluent to extract ART, DHA and QRT.
Results: The MRM transitions of ART, DHA, QRT and ATM were found to be 407.2â??261.0, 307.1â??261.0,
301.1â??151.0 and 283.2â??265.05, respectively. Collision energies used were -17, - 12, 22 and -15ev for
ART, DHA, QRT and ATM, respectively. The extraction recoveries of ART, DHA and QRT were found to be
80.5â??88.69%, 76.7â??81.52% and 89.76-94.89%, respectively. The lower limit of quantification (LLOQ) was
1 ngâ??mLâ??1 for ART and DHA whereas for QRT it was 50 Ng.mL-1.
Conclusion: The developed LC-MS/MS method is simple, sensitive and rapid. Moreover, the proposed
method can be successfully applied for the simultaneous analysis of ART, DHA and QRT in rat plasma for
the pharmacokinetics studies