ISSN: 2168-958X
+44 1478 350008
Gleysin Cabrera, Victor Salazar, Raquel Montesino, Yanet T�¡mbara, Weston B Struwe, Evelyn Leon, David J Harvey, Antoine Lesur, M�³nica Rincon, Bruno Domon, Milagros Mendez, Madelon Portela, Annia Gonzalez-Hern�¡ndez, Ada Triguero, Rosario Duran, Ulf Lundberg, Eva Vonasek and Luis Javier Gon
Center for Genetic Engineering and Biotechnology, Cuba
Venezuelan Institute for Scientific Research, Venezuela
Universidad de Concepci�³n, Chile
University of Oxford, UK
Luxembourg Clinical Proteomics Center, Luxembourg
Unidad de BioquÃ?Âmica y ProteÃ?³mica AnalÃ?Âticas, Uruguay
Posters & Accepted Abstracts: J Glycobiol
Contact with the urticating setae from the abdomen of adult females of the neo-tropical moth Hylesia metabus gives rise to an urticating dermatitis, characterized by intense pruritus, generalized malaise and occasionally ocular lesions (lepidopterism). The setae contain a pro-inflammatory glycosylated protease homologous to other S1A serine proteases of insects. Deglycosylation with PNGase F in the presence of a buffer prepared with 40% H2 18O allowed the assignment of an N-glycosylation site. Five main paucimannosidic N-glycans were identified, three of which were exclusively �±(1-6)-fucosylated at the proximal GlcNAc. A considerable portion of these N-glycans are anionic species sulfated on either the 4- or the 6-position of the �±(1-6)-mannose residue of the core. The application of chemically and enzymatically modified variants of the toxin in an animal model in guinea pigs showed that the pro-inflammatory and immunological reactions, e.g., disseminated fibrin deposition and activation of neutrophils are due to the presence of sulfate-linked groups and not on disulfide bonds as demonstrated by the reduction and S-alkylation of the toxin. On the other hand, the hemorrhagic vascular lesions observed are attributed to the proteolytic activity of the toxin. Thus, N-glycan sulfation may constitute a defense mechanism against predators.
Email: gleysin.cabrera@cigb.edu.cu