Journal of Chemical Engineering & Process Technology
Open Access

Studies on the use of genetically modified hyperthermophilic enzymes for the depolymerization of Saccharum officinarum biomass

3^rd International Conference on Chemical Engineering

October 02-04, 2017 Chicago, USA

Ali Nawaz

Government College University Lahore, Pakistan

Posters & Accepted Abstracts: J Chem Eng Process Technol

Abstract :

The elevated prices of oil, imminent shortage of traditional energy sources and environmental hazards has laid stress to take into consideration the use of bioethanol. The production of bioethanol contains different steps, most important being the saccharification to produce fermentable sugars that can later be converted into ethanol. In the present study, highly thermophilic cellulolytic enzymes i.e., endoglucanase, exoglucanase and �²-glucosidase produced in genetically modified mesophilic host were used for depolymerization of bagasse. Pretreated bagasse using alkaline peroxide was screened and yield of 26.64% was achieved using 150 units of endoglucanase, 300 units of exoglucanase and 600 units of �²-glucosidase added simultaneously in the reaction mixture at 80�°C after 3 hours. Substrate concentration of 0.75% (w/v) gave best hydrolysis rate of 29.19%. The percentage saccharification approximately increased up to 4 folds which is an important step forward for cost effective development of industrial process.