Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Amalia Tri Utami, Bogi Pratomo and Noorhamdani
State Islamic University of Maulana Malik Ibrahim Malang
Saiful Anwar General Hospital, Indonesia
Posters & Accepted Abstracts: Med Aromat Plants
Objective: To determine the effectiveness of extracts of black cumin seeds (Nigella sativa L.) as an antimicrobial against Salmonella typhi in vitro. And to determine minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) from extracts of black cumin seeds (Nigella sativa L.) against Salmonella typhi. Design: This experimental study used post-test only control group design with four time repetition. Step one was cultivating bacteria in liquid medium with various concentration of extract, that was 40%, 42.5%, 45%, 47.5% and 50% with two control, extract control and bacterial control. Results: The MIC (minimal inhibition concentration) was 45% concentration of extract. Step two was plating in NAP (nutrient agar plate) medium. The MBC (minimal bactericidal concentration) was 47.5% concentration of extract. The result of experiment showed different average of Salmonella typhi colony from every group. The result of the experiment was analyzed by one way ANOVA test. The hypothesis test of MBC showed significant differentiation, and then was continued with regression test. The study can be concluded that the addition of extract concentration showed decrease in the average of Salmonella typhi colony.
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