Journal of Nutrition & Food Sciences
Open Access

Study on bioavailability of 5-demethyl tangeretin and its acetylated derivative in PC-3 cells by HPLC with electrochemical detector

10^th World Congress on Nutrition & Food Sciences

May 29-31, 2017 Osaka, Japan

Jia-Lin Guo, Yin-Bo Chen, Hui-Yun Tsai, Shiming Li, Chi-Tang Ho and Yu-Kuo Chen

National Pingtung University of Science and Technology, Taiwan
Rutgers University, USA

Posters & Accepted Abstracts: J Nutr Food Sci

Abstract :

Tangeretin (TAN), a kind of polymethoxyflavones (PMFs) is found in citrus peels and has been proved to have many kinds of bioactivities including anti-inflammatory, anti-atherosclerosis and antitumor activities. Prostate cancer is universal malignant in male genital system. According to studies, 5-demethyltangeretin has hydroxyl group at the C5 position, and higher cytotoxicity than TAN for cancer cells, but the low solubility of 5-DTAN limits their availability in biological system. For the reason that we prepared 5-DTAN derivative (with acetyl group at the C5 position, 5-acetyltangeretin derivative; 5-ATAN), and hope to increase its solubility and bioavailability. The objective of this study is to investigate the anti-proliferation and cellular uptake of 5-ATAN in PC-3 human prostate cancer cells. Esterase present in the cells, therefor, 5-ATAN will be converted to 5-DTAN when 5-ATAN was uptaken into cells. We could use high-performance liquid chromatography lined electrochemical detector (ECD) to detect concentration of 5-DTAN within PC-3 cell. In MTT assay, it demonstrated that 5-ATAN significantly inhibit growth of PC-3 cells in dose- and time-depend manners. In condition of HPLC-ECD showed that 300 mV is the best volt, and retention time of 5-DTAN is 11.6 minutes, and linear regression is 0.1-10 �¼M. Moreover, the quantification and detection limits (LOQ and LOQ) of 5-DTAN were 7.16 and 1.79 �¼g/ml, respectively. In bioavailability test, intracellular concentration of 5-DTAN of 5-ATAN-treated group was significantly higher than those of 5-DTAN-treated group, which showed dose- and time-depend responses. Overall, the results indicate that 5-ATAN showed highest anti-proliferation activity than TAN & 5-DTAN in PC-3 cancer cells, and had greater bioavailability than 5-DTAN. 5-ATAN will be expected to have better oral bioavailability in vivo.

Biography :

Jia Lin Guo is currently pursuing his Master’s degree in Food Science from National Pingtung University of Science and Technology and has expertise in HPLC analysis of natural compounds.

Email: lazypapaman@gmail.com