Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Luca L Nazmi
Ain Shams University, Egypt
Posters & Accepted Abstracts: J Cell Sci Ther
Haematological malignancies represent approximately 7% of all malignant diseases. Acute myeloid leukaemia (AML) is an aggressive and fatal disease. AML treatment basically remained unimproved in the last 20 years, and depends upon induction of cytotoxic chemotherapy. An average less than 30% of AML patients survive for long-term. Mesenchymal stem cells (MSCs) are currently being investigated for an ever-expanding number of clinical indications based on their tissue-regenerative, immunomodulatory, and anti-inflammatory effects. The aim of the present work is to detect the effect of human umbilical cord blood-derived mesenchymal stem cells (HUCB-MSCs) on the expression of the leukaemic inhibitory factor gene (LIF) and on interleukin-10 (IL-10) in AMLpatients. The MSCs were separated from HUCB, and co-cultured with samples collected from peripheral blood (PB) of AML-insulted adults prior to chemotherapy. The expression of LIF gene and the IL-10 level were measured using the real-time polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA) techniques, respectively before and after the co-culture in order to evaluate the immunomodulatory effect of the MSCs. The present study revealed that the group of AML cells co-cultured with HUCB-MSCs showed a significant increase in the expression level of LIF gene compared with the untreated group. The group of AML cells co-cultured with MSCs showed a significant decrease in the IL-10 concentration compared to that of the untreated group. Our data demonstrated that co-culture of AML with MSCs represents a simple approach to inhibit leukaemic cells in vitro. monla20002000@yahoo.com