Immunome Research
Open Access

The human antibody 3E1 targets a novel epitope of the HA stem region to neutralize the H1N1 and H5N6 influenza A viruses

8^th Molecular Immunology and Immunogenetics Congress

March 20-21, 2017 Rome, Italy

Bing Sun, Wenshuai Wang, Xiaoyu Sun, Yanbing Li, Hualan Chen and Jianping Ding

Shanghai Institutes for Biological Sciences- CAS, China
Institut Pasteur of Shanghai- CAS, China
Harbin Veterinary Research Institute- Chinese Academy of Agricultural Sciences, China

Posters & Accepted Abstracts: J Immunome Res

Abstract :

As influenza A viruses remain a major threat to human health worldwide, the discovery of broadly neutralizing monoclonal antibodies that recognize conserved epitopes would facilitate the development of antibody-based therapeutic strategies. In this study, we show that a novel VH4-4-encoded human mAb named 3E1 could neutralize H1 and H5 subtype viruses in vitro and protect mice against the H1N1 and H5N6 viruses by inhibiting the low pH-induced conformational rearrangement of hemagglutinin (HA), hence blocking membrane fusion. The crystal structures of 3E1 Fab in complex with HA of two H1N1 strains reveal that 3E1, with both heavy and light chains, binds to a novel epitope of the conserved HA stem region, comprising parts of the fusion peptide, the F subdomain and the outermost �²-strand preceding helix A. Together, these data suggest the potential of 3E1 as a therapeutic drug and new strategies for the development of antiviral drugs and universal vaccines against H1 and H5 subtype viruses.

Biography :

Email: bsun@sibs.ac.cn