Journal of Fundamentals of Renewable Energy and Applications
Open Access
ISSN: 2090-4541
+44 1300 500008
ISSN: 2090-4541
+44 1300 500008
Lijie Zhang, Haiyan Pei*, Zhigang Yang, Liqun Jiang, Qingjie Hou, Zhen Xie, Yizhen Li
Shandong University, China
Scientific Tracks Abstracts: J Fundam Renewable Energy Appl
Microtubules have been verified to be able to regulate the precursors in microalgal cells. With the aim of testing the assumption that microtubule disruption could regulate precursors for complex organic matters and redirect carbon flow to promote lipid accumulation, Chlorella sorokiniana SDEC- 18 was pretreated with different concentrations of oryzalin. Strikingly, microalgae, pretreated with 1.5 mM of oryzalin, accumulated 41.06% of lipid content which was 1.4 times higher than that without pretreatment, attributed to the cellular cross-talk in carbon partitioning induced by microtubule destruction. Furthermore, complete extraction of lipid was achieved after only one extraction steps in microalgae pretreated with 1.5 mM of oryzalin, that was because microtubules were depolymerized with oryzalin pretreatment, the cellulose synthase would be removed from membrane, and cellulose biosynthesis was then blocked, which enhanced cell fragility and thus easier to be broken. This study provided an important advance towards observation of microtubules in microalgae through immunocolloidal gold techniques combined with TEM, and microtubule destruction inducing efficient lipid accumulation and increased cell fragility expanded connotation of metabolic regulation by microtubules. (Up to 250 words)
Keywords: Microalgae; Microtubule destruction; Lipid accumulation; Lipid extraction; Cell fragility.
Lijie Zhang has completed her PhD at the age of 27 years from Shandong University. She has published 4 papers as the first author (Average impact factor was 6.06) in top journals and has completed 2 invention patents. Besides, she has gone to Miami, USA and Copenhagen, Denmark to attend international conferences in 2017 and 2018.