Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
+32 25889658
ISSN: 2155-9600
+32 25889658
Ala�a Al-Bakheit, Antonietta Melchini, Maria Traka and Richard Mithen
Al-Balqa� Applied University, Jordan
Institute of Food Research, UK
Posters & Accepted Abstracts: J Nutr Food Sci
Acylcarnitines are intermediate compounds in lipid metabolism that accumulate in plasma as a result of metabolic dysregulation. Dietary intake of sulforaphane, a bioactive compound delivered by broccoli consumption, is able to significantly reduce plasma levels of acylcarnitines in subjects with moderate risk of developing cardiovascular diseases. Palmitoylcarnitine (palcar), a C16:00 acylcarnitine, is an intermediate in mitochondrial fatty acid oxidation, accumulate in plasma as a result of insufficient integration of fatty acid �² oxidation with TCA cycle due to high levels of oxidative stress which inhibits TCA cycle enzymes. As it has been reported that metabolism is disrupted in prostate cancer, in this study we found that palcar increased significantly in human prostate cancer tissues compared to non-cancerous tissues. We then investigated the potential biological consequences of high levels of palcar in in vitro. We found that palcar (50 �¼M) induces rapid Ca2+ influx only in the human prostate cancer PC3 cells, but not in the human prostate non-cancerous (PNT1A and BPH-1) and DU145 cells that are moderately metastatic compared to PC3 cells. Palcar effects on Ca2+ influx were similar to those induced by dihydrotestosterone (DHT), an androgen associated with prostate cancer progression. We also observed a significant induction of interleukin-6 cytokine in 50 �¼M palcar-treated PC3 cells (pâ�¤0.05). These data suggest that palcar could be considered a biomarker for the metabolic dysfunction associated with prostate cancer and provide evidence of palcar pro-inflammatory effect in highly metastatic prostate cancer cells which may be related to a [Ca2+]i-dependent mechanism of action.
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