Journal of Clinical and Cellular Immunology
Open Access

Toxoplasma gondii tachyzoites induce NETosis in donkeys

Conference Series LLC Joint International Event on 5^th European Immunology & Innate Immunity

July 21-23, 2016 Berlin, Germany

Sami Gokpinar, Kader Yildiz, Neslihan Sursal, Cahit Babur and Ahmet Kursat Azkur

Kirikkale University, Turkey
Ankara University, Turkey
Turkiye Halk Sagligi Kurumu, Turkey

Posters & Accepted Abstracts: J Clin Cell Immunol

Abstract :

This work was supported by the grand from TUBITAK (Project no: TOVAG 214O288)

Toxoplasma gondii infected a lot of intermediate hosts including equids. Serum antibodies against to T. gondii have been found in donkeys in the world. Neutrophil extracellular traps (NETs) are one of the major fighting mechanisms of polymorph nuclear neutrophils (PMN) against pathogens. NETs provide extracellular entrapment and killing of pathogens including parasites in organism. The aim of the study was to detect NETs formation of polymorph nuclear neutrophils (PMN) of donkey encounter with T. gondii tachyzoites. For the first time, we demonstrate extracellular traps released from donkey PMN after confronted with T. gondii tachyzoites in vitro. Myeloperoxidase, neutrophil elastase and histones (H3) were observed in fine NETs structures triggered by T. gondii using fluorescence microscopy. Fluorometric analysis revealed that NETs amount increased depending on incubation time.

Biography :

Sami Gokpinar has completed his PhD Education and Postdoctoral studies in Kirikkale University, Turkey. He has studied Parasitology at the University Kirikkale, Turkey.