Journal of Antivirals & Antiretrovirals
Open Access

Transcriptome analysis of rice infected with rice viruses reveals the relation between the symptoms and responses of host

International Conference and Exhibition on VIROLOGY

5-7 September 2011 Baltimore, USA

Shoshi Kikuchi

Scientific Tracks Abstracts: JAA

Abstract :

Using the 44k microarray system of rice, we have studied the gene expression profi les of rice infected with nine rice viruses, including RDV (Rice dwarf virus), RBSDV (Rice blackstreaked dwarf virus), RRSV (Rice ragged stunt virus), RGDV (Rice gall dwarf virus), RSV (Rice stripe virus), RGSV (Rice grassy stunt virus), RTYV (Rice transitory yellowing virus), RTSV (Rice tungro spherical virus) and RTBV (Rice tungro bacilliform virus). Th ree strains of RDV (S: severe, D-84: middle, O: weak) show the diff erence of induction of diff erence related genes mediated by the jasmonic acid pathway and repression of the genes related with morphogenesis related genes such as the cellular division. RGSV induces the tillering of shoot aft er infection in rice. Th is interesting symptom is mediated by the repression of the receptor genes of trigolactone (SL) and deactivation of bioactive Gibberellins. RTSV helps the infection of RTBV to induce the Tungro disease, but RTSV alone cannot induce severe symptoms in its infection. By using the host of eIF4G7ch mutant, that cannot support the growth of RTSV, gene expression analysis showed the diff erence of host response when virus propagation was completely achieved and incompletely terminated. We have also compared the global gene expression profi les of nine virus-infection with other environmental stresses such as drought, temperature, salinity etc and will discuss how they are similar and what kinds of diff erence are there.

Biography :

Shoshi Kikuchi has completed his Ph.D at the age of 27 years from the University of Tokyo and got the position in Plant Virus Institute, then the institute was reorganized to the National Institute of Agrobiological Sciences. He has made the massive collection of the rice full-length cDNA clones with RIKEN and FAIS research groups and also established the rice microarray systems. By making the gene family analysis of transcription factor genes such as NAC and AP2/EREBP, he has been contributed the functional annotation of rice genes. The work presented in this symposium is the product of BRAIN project (2007~2012).