Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Nega Berhane, Kibrenesh Tegenaw, Wagaw Sendeku, Mulugeta Aemero and Desalgne Mengesha
University of Gondar, Ethiopia
Scientific Tracks Abstracts: J Clin Cell Immunol
Background & Aim: Plasmodium falciparum is the most dangerous species of plasmodium parasites in terms of lethality and morbidity. In different studies, polymorphisms in the tumor necrosis factor alpha (TNF-�±) gene have been associated with increased susceptibility to mild malaria infection and severe malaria. The aim of this study was to determine the frequency of TNF-�±-308 G > A gene polymorphism in P. falciparum malaria infected patients living in Dembiya Woreda, North Gondar, North West Ethiopia and to assess the effect of TNF-�±-308 gene polymorphism and different demographic factors on the risk of malaria infection. Methods & Results: Two hundred blood samples were collected from November to December 2014 from clinically confirmed P. falciparum malaria patients (n=100) and from P. falciparum seronegative individuals (n=100) who live in the study area. TNF-�±-308 G > A polymorphism was detected using PCR- RFLP techniques. The mean age of P. falciparum malaria patient study subjects was 23.2�±8.36 years old. Age (P=0.000) and occupation (P=0.046) were associated risk factors for malaria infection at 95% CI. The allele frequency in malaria patient study subjects was 0.92 for TNF-�±-308G (TNF-1) and 0.08 for TNF-�±-308A (TNF-2). The distribution of TNF-�±-308 genotypes in cases (P=0.065) and controls (P=0.677) were consistent with the Hardy-Weinberg equilibrium. Conclusion: There was no statistically significant association between TNF-�±-308 genotypes and malaria infection (P=0.616). Further studies with large number of sample size and assessment in different malaria endemic areas of the country are warranted for generalization.
Nega Berhane has research expertise in Biotechnology, Cancer Research, Cell Biology. He had completed his Ph.D. in Biotechnology from University of Gondar, Ethiopia; He has published many papers in reputed journals and attended in many national conferences