Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Fang-qiu Li, Xiu-juan Shang, Xiao Chen, Yu-an Hu and Li-ning Shi
Nanjing University, China
Posters & Accepted Abstracts: J Clin Cell Immunol
Objective: The rapid elevation of IgG antibody against Candida enolase has been observed in the patients at early stage of invasive candidosis. The aim of the study was to build a comprehension of the rapid increase of specific IgG titers against dominant antigens (such as enolase) at the early stage of invasive Candida infection resulted from humoral immune anamnestic response associated with previous Candida colonization. Methods: The oral Candida colonization mouse model was established, and the immuno-competent and immuno-compromised Candida colonized mice were challenged by intraperitoneal injection of Candida spore. The numbers of enolase-specific memory B-cells in the spleen were measured by ELISPOT and compared with the levels of specific IgG, IgM and IgA antibodies in the peripheral blood. Results: The burst of enolase-specific memory B cells was detected in both immuno-competent and immuno-compromised mice at day 7 post-invasive infection; which was followed by a strong increase in specific antibody titre in the Candida colonized mice. The Eno-IgG antibody was positively correlated with the antigen specific Bm (r=0.737, P<0.01). Conclusion: It was confirmed that the Candida colonization associated with anamnestic response of IgG against dominant antigen at early stage of invasive candidosis and the rapid elevation of specific-IgG would suggest a diagnosis of invasive infection.
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