Journal of Pharmaceutical Care & Health Systems
Open Access
ISSN: 2376-0419
+44 1300 500008
ISSN: 2376-0419
+44 1300 500008
Jorge Parodi
Universidad Catolica de Temuco, Chile
Posters-Accepted Abstracts: J Pharma Care Health Sys
Didimosphenia geminata (â??didymoâ?) has become a powerful and devastating river plague in Chile. A system was developed in D. geminata channels with the purpose of evaluating the effects of water polluted with didymo on the activation of Atlantic salmon (Salmo salar) spermatozoa. Results indicate that semen, when activated with uncontaminated river water had an average time of 60?±21 s. When using Powermilt, (a commercial activator), times of 240?±21 s are achieved, while rivers contaminated with D. geminata achieve a motility time of 30?±12 s. Interestingly enough, the kinetic parameters of VSL, VCL and VAP showed no significant changes under all of the conditions. Furthermore, the presence of D. geminata reduces activation time of the samples as the cells age, indicating increased effects in spermatozoa that are conserved for more than 5 days. D. geminata has antioxidant content, represented by polyphenols. 200 ppm of polyphenol was obtained, in this study, per 10 grams of microalgae. Spermatozoa exposed to these extracts showed a reduction in mobility time in a dose dependent manner, showing an IC50 of 15 ppm. The results suggest an effect on spermatozoa activation, possibly due to the release of polyphenols present in contaminated rivers, facilitating the alteration of sperm motility times, without affecting the viability or kinetics of the cells. These findings have important implications for current policy regarding the control of the algae. Current control measures focus on the number of visible species, and not on the compounds that they release. As shown in this study, these released compounds also have a problematic effect on salmon production.
Email: jparodi@uct.cl