Journal of Glycobiology
Open Access

World glycobiology

Glycobiology World Congress

August 10-12, 2015 Philadelphia, USA

Munira Shahbuddin

Posters-Accepted Abstracts: J Glycobiol

Abstract :

Konjacglucomannan (KGM) of different molecular weights (MW) cultivates different glycosylation sites on cell surface
which affect cellular biological functions and metabolism. Mannosylated structures can potentially illicit immune
recognition responses via mannose receptor. In this study, we demonstrated the effect of supplementation of �²-mannanase
treated KGM with different MW distributions in culture media on mammalian cell subjected to shear stress and hypoxia
conditions. The non-modified KGM and with higher MW stimulated fibroblast and adipose stem cell proliferation. KGM
also helped to reduce detrimental effect of shear stress and hypoxia by 50%. Inverse relationship was observed in HaCaT and
keratinocytes, which highlighted the differences in cell surface receptors and the importance of structure-activity relationship
to bridge the gap between characterizations and biological properties.