Journal of Pharmaceutical Care & Health Systems
Open Access

Xanthotoxin, a furanocoumarin compound expresses anti-inflammatory effects through suppression of iNOS, COX-2, TNF-α, and IL-6 via AP-1, NF-κB, and JAK-STAT inactivation in RAW 264.7 cells

10^TH Asia-Pacific Pharma Congress

May 08-10, 2017 Singapore

Seung-Bin Lee, Woo Seok Lee, Ji-Sun Shin, Dae Sik Jang and Kyung Tae Lee

Kyung Hee University, South Korea

Posters & Accepted Abstracts: J Pharma Care Health Sys

Abstract :

Xanthotoxin has been reported to possess skin-protective and anti-oxidative properties. However, anti-inflammatory property has not been studied to date. Therefore, we investigated the role that xanthotoxin plays on anti-inflammatory activity as well as its underlying molecular mechanisms in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. In LPS-induced macrophages, xanthotoxin was found to inhibit nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor (TNF-�±), and interleukin-6 (IL-6) in a concentration-dependent manner. It also suppressed the LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression at the protein level and iNOS, COX-2, TNF-�±, and IL-6 at the mRNA level. Molecular mechanism shows that xanthotoxin attenuated the LPS-induced transcriptional and DNA-binding activity of activator protein-1 (AP-1), and this was associated with a decrease in the phosphorylation of c-Fos instead of c-Jun. It played a suppressive effect on the transcriptional and DNA-binding activity of nuclear transcription factor kappa-B (NF-�ºB) through the inhibiting of p65 nuclear translocation. In addition, the LPS-induced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogenactivated protein kinase (MAPK) was found to be suppressed by xanthotoxin. Taken together, these results indicate that xanthotoxin decreased NO, PGE2, TNF-�±, and IL-6 production through downregulation of NF-�ºB, AP-1, and JAK/STAT signaling pathways in LPS-induced RAW 264.7 macrophages.

Biography :

Seung-Bin Lee is a student at Kyung Hee University in South Korea and has been intensively studied on screening anti-inflammatory effect among various natural product derived compounds. In an idea to alleviate these tendency, he has been investigated the underlying molecular mechanism of several drugs which elicit significant decrease of inflammatory endpoints such as nitric oxide, prostaglandin E2, and pro-inflammtory cytokines.

Email: bengood224@naver.com