Advancements in Genetic Engineering
Open Access

RFLP

RFLP (often pronounced "rif lip", as if it were a word) is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives.

Each organism inherits its DNA from its parents. Since DNA is replicated with each generation, any given sequence can be passed on to the next generation. An RFLP is a sequence of DNA that has a restriction site on each end with a "target" sequence in between. A target sequence is any segment of DNA that bind to a probe by forming complementary base pairs. A probe is a sequence of single-stranded DNA that has been tagged with radioactivity or an enzyme so that the probe can be detected. When a probe base pairs to its target, the investigator can detect this binding and know where the target sequence is since the probe is detectable. RFLP produces a series of bands when a Southern blot is performed with a particular combination of restriction enzyme and probe sequence.

Related journals of RFLP

Gene Technology, Genetic Disorders & Genetic Reports Hybrid, Genetic Syndromes & Gene Therapy, Hereditary Genetics: Current Research, Epigenetics and Human Health, European journal ofgenetics in society : an ethical approach togenetics, Genetics and Breeding, Genetics and EpiGenetics, Human Ontogenetics, Immunology and Immunogenetics Insights.