Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

Bianca Malecki

Publications

• Research Article
  Routing of Biomolecules and Transgenes' Vectors in Nuclei of Oocytes
  Author(s): Marek Malecki and Bianca MaleckiMarek Malecki and Bianca Malecki
  
  The molecular architecture of Nuclear Pore Complexes (NPCs), as well as the import and export of molecules through them, has been intensively studied in a variety of cells, including oocytes. However, the structures and mechanisms, involved in the transport of molecules beyond the NPCs, remained unclear, until now. The specific aim of this work was, therefore, to determine, if there exist any intranuclear structures in continuum with the NPCs. This information could help in explaining the mechanisms, which propel the distribution of biomolecules and vectors inside the cell nuclei. To attain this aim, we used rapid cryo-immobilization to capture molecular processes of living cells with millisecond resolution. We pursued molecular imaging, including electron energy loss spectroscopy and energy dispersive x-ray spectroscopy, to reveal structures with nanometer spatial re.. View More»
  DOI: 10.4172/2165-7491.1000108

  Abstract PDF

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