Journal of Clinical & Experimental Dermatology Research
Open Access
ISSN: 2155-9554
+44 1478 350008
ISSN: 2155-9554
+44 1478 350008
Toni Febriyanto
Universitas Gadjah Mada, Indonesia
Posters & Accepted Abstracts: J Clin Exp Dermatol Res
Background: Ultraviolet (UV) exposure causes photo aging by inducing the expression of matrix metalloproteinase-1 (MMP- 1) expression. Snail Achatina fulica is one of the most extensively studied snails due to its economic, ecological and medical importance. Moreover, the slime is well-known for its wound-healing and anti-bacterial properties. Objective: The research is aimed to investigate whether snail slime of Achatina fulica exhibits anti-photoaging properties by inhibiting the expression of MMP-1. Method: Fifty snails were stimulated by a 9V electricity source for the extraction of slime. The extracted slime was then converted into powder form by the freeze-drying process. The slime powder was dissolved in dimethyl sulfoxide (DMSO) to desired concentrations. At the end of the experiment, the MMP-1 expression in different fibroblast groups was assessed by qPCR. Results: Administration of snail slime has proven to lower MMP-1 expression compared to the positive control group without snail slime. However, administration of snail slime at various concentrations did not significantly produce different results. MMP-1 expression level is slightly lower in slime group compared to the Ellicina cream group at the concentration of 25 and 50 μg/mL. Administration of Ellicina cream at the concentration of 100 μg/mL has shown to lower MMP-1 expression compared to slime group at any concentration. Conclusions: Snail slime of Achatina fulica reduces MMP-1 expression level on UVB-induced fibroblast culture. The potency in inhibiting MMP-1 expression level between Achatina fulica slime and Ellicina cream is not much different at certain concentrations.
E-mail: toni.tfeb@gmail.com