Journal of Medical Diagnostic Methods
Open Access

Biofunctionalized carbon nanotubes platform for biomedical application

International Conference on Medical Devices

September 21-22, 2015 Orlando, USA

K Kamil Reza1, Saurabh Srivastava1, 2, Surendra K Yadav1 and A M Biradar1

1National Physical Laboratory, India
2Banaras Hindu University, India

Posters-Accepted Abstracts: J Med Diagn Meth

Abstract :

We have fabricated the chemically functionalized multi-walled carbon nanotubes (MWCNT) electrophoretically deposited onto indium tin oxide for urea detection. Urease and glutamate dehydrogenase are attached to MWCNT/ITO electrode surface covalently using ethyl-dimethy laminopropyl-carbodiimide and N-hydroxysuccinimide coupling chemistry. Excellent electrochemical properties of MWCNT and covalent functionalization of dual enzyme improve biosensor stability and sensitivity. The electrochemical response studies of the proposed biosensor exhibits wide detection range (0.83-24.9 mM), low detection limit (1.3 mM) and higher sensitivity of 4.67 �¼ AmM-1cm-2. The low magnitude (0.274 mM) of the apparent Michaelis-Menten constant (Km) value indicates high enzymatic affinity of Urs-GLDH enzymes to MWCNT/ITO electrode surface.

Biography :

Email: kamilreza@gmail.com