Journal of Physical Chemistry & Biophysics
Open Access
ISSN: 2161-0398
+44 1478 350008
ISSN: 2161-0398
+44 1478 350008
Nádia Carolina Teixeira Marques1, Natalino Lourenço Neto1, Ana Paula Fernandes1, Bianca Zeponi Fernandes Mello1, Camila Oliveira Rodini2, Vivien Thiemy Sakai3, Thiago Cruvinel Silva1, Maria Aparecida Andrade Moreira Machado1, Thais Marchini Oliveira1
Scientific Tracks Abstracts: J Phys Chem Biophys
The aim of this study was to evaluate the effects of Low-Level Laser therapy (LLLT) on dentin matrix protein-1 (DMP- 1) expression in pulp tissue repair of human primary teeth. Twenty mandibular primary molars were allocated into the following groups: Buckleyâ??s Formocresol (FC); Calcium Hydroxide (CH); LLLT + CH and LLLT + Zinc/Oxide Eugenol (ZOE), and received pulpotomy treatment. The teeth at the regular exfoliation period were extracted and further processed for histological evaluation and immunolocalization of DMP-1. An established score system was used to analyze the dentin pulp complex, and for the immunolocalization of the DMP-1 a descriptively analysis was carried out. Statistical analysis was performed by Kruskal-Wallis and chi-square test (p<0.05). LLLT+CH group presented the lowest degree of pulpal inflammation (p=0.0296) compared to FC group. The highest rate of hard tissue barrier (p=0.0033) and odontoblastic layer (p=0.0033) was present in CH group than in FC and LLLT+ZOE groups; furthermore, CH group showed higher rates for dense collagen fibers (p=0.0095) in comparison with both groups. In contrast with LLLT+ZOE and LLLT+CH groups, FC revealed the highest incidence of internal resorption (p=0.0142). Immunohistochemistry analysis showed DMP-1 immunopositivity in CH and LLLT+CH groups, indicating the reparative and bio-inductive capacity of these materials. No immunostaining for DMP-1 was observed both in FC and LLLT+ZOE groups. According to this study, Low-Level Laser Therapy preceding the use of calcium hydroxide exhibited satisfactory histological response and bio-inductive activity by the expression of DMP-1 on pulp tissue healing of human primary teeth.
Nádia Carolina Teixeira Marques graduated in Dentistry from Federal University of Alfenas â?? UNIFAL-MG. At the age of 24 years became MSc in Pediatric Dentistry at Bauru School of Dentistry, USP - University of São Paulo. Currently acts as PhD student from Bauru School of Dentistry, USP - University of São Paulo. She has published 10 papers in scientific journals, besides serving as periodic reviewer. Areas of Expertise: Pediatric dentistry; Pulp therapy; Laser Therapy.