Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Narayan C. Rath
Scientific Tracks Abstracts: J Clinic Toxicol
T hiram is a general use dithiocarbamate pesticide. It causes tibial dyschondroplasia, a growth plate cartilage broadening defect in poultry caused by the accumulation of nonviable chondrocytes which lead to lameness. Since proteins play crucial roles in all aspects cell physiology including survival, growth, and differentiation, the objective of this study was to find whether thiram induces proteomic changes that may be consequential to the failure of endochondral bone development. Growth plate chondrocytes from proximal tibia of 2wk-old broiler chicks were cultured without or with a sublethal concentration of thiram (1�¼M) for 48 h and the cell protein extracts subjected to 2-D gel electrophoresis. Triplicate gels from each group were compared and statistically evaluated using Melanie software to find differentially expressed silver stained protein spots. Of total 72 identified spots 3 showed down-and 2 up- regulation in thiram treated group. In-gel trypsin digestion of these spots followed by mass spectrometry could identify only two down-regulated protein spots, namely a heat shock protein 70 (HSP70) and GalE protein. The up-regulated proteins included Serpin H1 precursor protein or HSP47, and a NMRA-like protein-1. HSP70 is cytoprotective against cellular stress and GalE involved in proteoglycan metabolism. NMRAL protein acts as a redox sensor protein and HSP47 binds collagen and regulates its quality and degradation. While, the exact significance of the changes in the above proteins are not clear, it appears that thiram induces cellular stress, modulating heat shock protein portals affecting both functional and structural integrity of chondrocytes which lead to their apoptosis impairing growth plate development.
Narayan Rath has over 40 years of research experience. After receiving Ph. D from University of Delhi, India, he did postdoctoral work at CNRS, France, and at the National Institutes of Health, Bethesda. He has published over 100 peer reviewed papers.