Journal of Antivirals & Antiretrovirals
Open Access
ISSN: 1948-5964
+44 1300 500008
ISSN: 1948-5964
+44 1300 500008
Palanee Ammaranond
Chulalongkorn University, Bangkok, Thailand
Posters & Accepted Abstracts: J Antivir Antiretrovir
Plasma samples were collected for HIV viral load and drug resistance testing. Cold chain for transportation of plasma samples is needed. For more convenient, Dried Blood Spot (DBS) is an alternative way to collect specimen because it is easy for transportation from rural area. However, the cost of this filter paper is still expensive. HIV-1 viral load and HIV-1 drug resistance testing were measured. Among these three filter papers, NM was not capable of absorbing blood samples while Whatman 903 and CFSP were quality acceptable. Then, both of these two filter papers were evaluated. The measurement of viral load testing on both filter papers was no significantly differences in all ranges of viral load level (P 0.05, R2 = 0.98). For drug resistance testing, all resistance mutations on NRTI, NNRTI and PI were detected when compared with gold standard method. DBS provided acceptable quantitative precision and accuracy data. For a novel membrane substrate card, CFSP, was compatible prototype DBS card employs a with Whatman 903 filter paper. The described CFSP works well for HIV viral load and drug resistance assay. Our novel DBS card approximately costs 0.1 US dollars compared to $3 for a commercially available used. In Thailand, the burden of cost for treating HIV-infections is high not only for the average citizen but the country�s health care systems. Therefore, a low costing and yet effective DBS card for HIV-1 viral load and drug resistance is a practical and viable solution for resource-limited countries.
Email: palanee@gmail.com