Chemotherapy: Open Access
Open Access
ISSN: 2167-7700
ISSN: 2167-7700
Maria Tereza Cartaxo Muniz
Posters-Accepted Abstracts: Chemotherapy
The Human Papilloma Virus (HPV) has been extensively studied due to its carcinogenic potential, related to the integration of viral DNA into the host genome. About 120 different types of HPV have already been described based on the heterogeneity of genome. Among the high-risk types, also called oncogenic, the most common is HPV-16, accounting for about 60-70% cases of cervical cancer associated with viruses. In addition to carcinoma of the cervix, HPV has been linked to several cancers, including retinoblastoma. This work aims to verify the activity of HPV by different approaches, as the clastogenic action and viral protein expression analysis on samples of children from the city of Recife, Pernambuco, Brazil, evaluating this activity by Immuno-histochemistry techniques and in situ hybridization. The biological material for analysis was peripheral blood and retinoblastoma tumor samples. Ten children with retinoblastoma were evaluated. Viral detection was performed by PCR with specific primers for the HPV types 16 and 18. HPV was in eight children with retinoblastoma, being HPV-16 the most frequent. By Immunohistochemistry was possible to evidence the presence of viral proteins E1^E4 (antibody anti-HPV-16), L1 (anti-HPVs antibody 1, 6, 11, 16, 18 and 31) and E6 (antibody anti-HPV-16 and HPV-18) in waxed tissue in various segments of the eye, confirming the viral activity in the sample. The data are discussing the presence and expression of oncogenic HPV in retinoblastoma and the evidences of damage in the DNA of the host as a consequence of the presence and viral activity.