Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Richard Ortega
AcceptedAbstracts: J Clinic Toxicol
R ecent data suggest that the Golgi apparatus plays key roles in the homeostasis and detoxification of manganese. Manganese is an essential trace element but when high exposure conditions occur manganese induces neurological symptoms in human. Manganese is also suspected to be an environmental risk factor in the aetiology of Parkinson?s disease. However, the mechanisms regulating manganese homeostasis and detoxification in mammalian cells are largely unknown. Owing to the development of synchrotron radiation X-ray nano-chemical imaging we revealed the specific accumulation of manganese in the Golgi apparatus of dopaminergic cells in culture. At both physiological and subcytotoxic concentrations of manganese, we found that manganese was essentially located within the Golgi apparatus. At cytotoxic concentration of manganese, we found a large increase of manganese content in the cytoplasm and the nucleus of dopaminergic cells. Similarly, if Golgi apparatus is altered using brefeldin A, manganese reaches the nucleus and cytoplasm in higher content. The accumulation of manganese in the Golgi apparatus could have a preventing effect because manganese could be removed by exocytosis. However, vesicular trafficking could be disturbed by high concentrations of manganese leading to neuronal cell death. The potential mechanisms involving the role of Golgi apparatus alteration in neurological disorders triggered by manganese will be discussed
Richard Ortega has completed his Ph.D in Biophysics at the age of 24 years from Bordeaux University in France and postdoctoral studies from Hahn Meitner Institute, in Berlin, Germany, and at The Hospital for Sick Children, Dept of Biochemistry Research, Toronto, Canada. He is research director at CNRS (French National Scientific Research Center) and head of IPCV team (Interface Physics & Chemistry for Life Sciences) at CENBG, Bordeaux University, France. He has published more than 60 papers in reputed journals and serving as an editorial board member of Journal of Environmental Analytical Toxicology