Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Krocilova Nikola, Bacakova Lucie, Parizek Martin, Havlikova Jana, Motarjemi Hooman, Molitor Martin, Gabor Roman and Marvan Jaroslav
Institute of Physiology of the Czech Academy of Sciences, Czech Republic
Bulovka Hospital in Prague, Czech Republic
VUHZ Joint-Stock Co., Czech Republic
Medin Joint-Stock Co., Czech Republic
Posters & Accepted Abstracts: J Cell Sci Ther
Adipose-derived stem cells (ASCs) are of a great importance for bone tissue engineering and have many advantages e.g. are available in higher amounts than other stem cells. We compared ASCs obtained by liposuction under two pressures-200 mmHg (ASCs-L) and -700 mmHg (ASC-H). After isolation was the number ASCs-H higher than the number of ASCs-L. ASCs-H also reached higher population densities. ASCs were then characterized by flow cytometry for specific cell surface markers. We found out that ASCs-L were more heterogeneous. Titanium has good mechanical and biocompatible properties and Ti-6Al-4V alloy is currently used for bone replacements. The adhesion and growth of ASCs were studied on Ti-6Al-4V samples either unmodified sample K (Ra=0.28) or modified samples A (Ra=0.2), B (Ra=0.1) and C (Ra=0.08). On day 1, the largest cell spreading areas were on A and K and the smallest on B. On day 3, ASCs-H reached the highest population density on B. On day 7, the numbers of ASCs-H evened out on all modified samples and were higher than on non-modified. ASCs-H looks better for fast proliferation. Finally we studied osteogenic differentiation of ASCs on Ti-6Al-4V samples, where we measured the intensity of fluorescence of collagen I, alkaline phosphatase and osteocalcin. We observed that ASCs in osteogenic medium produced higher amounts of osteogenic markers than in standard medium, especially on A and K samples. We are planning to evaluate mineralization of extracellular matrix, to measure osteogenic markers by PCR and ELISA and to stimulate the differentiation by vibrational stress.
Krocilova Nikola has studied her Bachelor’s degree at the Palacky University in Olomouc, focused on cell and molecular biology. She is interested in the theme of minimal residual disease in breast cancer. Presently she is studying her Master’s degree at the Charles University in Prague with the specialization on cell and developmental biology. She has employed at the Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, where she is working on her Diploma with the project entitled Application of the stem cells in bone tissue engineering.
Email: n.kroci@seznam.cz