Journal of Cell Science & Therapy
Open Access

ICAM-1 expression by skeletal muscle cells augments stages of myogenesis

3^rd World Congress on Cell Science & Stem Cell Research

November 20-22, 2013 DoubleTree by Hilton Baltimore-BWI Airport, MD, USA

Qingnian Goh, Dearth C. L and F. X Pizza

University of Toledo, USA

Posters: J Cell Sci Ther

Abstract :

We previously reported that intercellular adhesion molecule-1 (ICAM-1), an important protein of the inflammatory response, was expressed by satellite cells and myofibers of hypertrophying muscles. As skeletal muscle cell expression of ICAM-1 contributed to the formation of regenerating fibers, as well as hypertrophy to mechanical overload, we speculate that ICAM-1 expression by skeletal muscle cells facilitates myogenesis. Therefore, the purpose of the current study was to establish phenotypic alterations associated with skeletal muscle cell expression of ICAM-1. Through stable transfection of C2C12 myoblasts with an ICAM-1 plasmid, we found ICAM-1 expression augmented stages of myogenesis in which myotubes are forming, adding nuclei, aligning, synthesizing proteins, and hypertrophying. As the cytoplasmic domain of ICAM-1 is capable of activating several intracellular signaling pathways, we selectively inhibited the function of the cytoplasmic domain with a cell permeable peptide to reveal underlying mechanisms involved in ICAM-1 mediated myogenesis. Here, we showed that the ICAM-1 cytoplasmic domain contributed to enhanced rates of protein synthesis via increased Akt/p70s6k signaling. Hence, our findings extend knowledge of the immunobiology of skeletal muscle cells by revealing a novel mechanism through which the inflammatory response facilitates myogenesis.

Biography :

Email:

qgoh@rockets.utoledo.edu