Transcriptomics: Open Access
Open Access
ISSN: 2329-8936
ISSN: 2329-8936
Seung-Won Park1, Jung-Won Choi1, Seong Ryul Kim2, Gwang-Ho Choi2, Kee-Young Kim2 and TaeWon Goo3
Accepted Abstracts: Transcriptomics
Decapentaplegic (Dpp) is the homolog of vertebrate BMP-2 and BMP-4 and functionally interchangeable BMPs. The Bombyx mori (B. mori) and Bombyx mandarina (B. mandarina) Dpp genes share genetic homology with human BMPs and Drosophila Dpp, but few studies have been executed to examine the functions of B. mori and B. mandarina Dpp and its function is not well understood. To date, there was also no reported splicing form of Dpp in silkworm. In this study, we investigated Dpp expression using synthesized cDNA from midgut tissue of B. mandarina by RT-PCR. Interestingly, lower band was discovered with band of full-length Dppc DNA and it was identified as novel splicing form that a part (333 bp) of B. mandarina Dpp was deleted through DNA sequencing analysis. In addition, we found that the delated part in the variant was a portion of proprotein region compared to human BMP-2 and 7 candidate single nucleotide variants (SNVs) were able to affect formation of novel splicing form using variant calling analysis. To the best of our knowledge, this is the first approach to address the novel splicing form of Dpp in B. mandarina which have been not found in B. mori. These results suggest that discovered splicing form of B. mandarina was degenerated in evolution process toward more advanced and domesticated B. mori.