Transcriptomics: Open Access
Open Access
ISSN: 2329-8936
ISSN: 2329-8936
Jong Eun Park, Mijeong Byun, Kyung Tai Lee, Young rok Yang, Mina Park, Dajeong Lim, Han Ha Chai, Han Tae Bang, Jong Hwangbo, Yang ho Choi and Yong Min Cho
National Institute of Animal Science, Republic of Korea
Gyeongsang National University, Republic of Korea
Posters & Accepted Abstracts: Transcriptomics
High temperature is prior factor in climate change following global warming. This envionmental factor decreases the growth and productivity in livestock. The Pekin duck, Anas platyrhynchos, is one of the most famous meat duck species worldwide. Thus, transcriptome and expression profiling data for pekin duck under heat stress are important resource for identifying genes and developing molecular markers. In this study, we compared transcriptome and expression profiles of 30 samples for different heating time (350C, 3 and 6 hours) and moisture (60% and 90%) with control. After trimming of reads, more than 30 milion reads and 96% of Q20 (per sample) were generated. From 17,160 transcripts (over 66% per sample), 3,576 transcripts with zero FPKMs (Fragments Per Kilobase Million) across more than 50% of all samples were excluded leaving 13,584 transcripts to be analyzed. After quantile normalization for remained transcripts, we identifed total 669 differentially expressed transcripts from 6 combinations of treatment (including control). The selection criterions were both absolute value of 2 fold change and significance of independent t-test (raw p-value<0.05). In further study, we will perform functional annotation, network analysis and experimetal validation to select candidate genes related with heat stress. This study will provide informative genes or markers that can be used to develope heat-tolerant duck strains.
Jong Eun Park has completed his PhD from Seoul National University. He is a Researcher at National Institute of Animal Science (NIAS), Rural Development Administration in Korea.
Email: jepark0105@korea.kr