Journal of Clinical and Cellular Immunology
Open Access

Influence of placenta soluble factors on receptor expression profile of JEG-3 trophoblast cells

Conference Series LLC Joint International Event on 5^th European Immunology & Innate Immunity

July 21-23, 2016 Berlin, Germany

Okorokova Larisa, Furaeva K N, Stepanova O I, Mikhailova V A, Koludarova L V, Selkov S A and Sokolov D I

D.O. Ott Research Institute of Obstetrics and Gynecology, Russia

Posters & Accepted Abstracts: J Clin Cell Immunol

Abstract :

Cells in the maternal-fetal interface secrete cytokines that regulate proliferation, migration and trophoblast invasion during pregnancy. The aim of the study was to evaluate the influence of factors secreted by human placenta during pregnancy on cytokine receptors expression of JEG-3 trophoblast cells. The research was conducted using the conditioned media of placentas obtained from healthy women with elective termination of pregnancy (9-11 w.g.) and placentas of women with pregnancy without complications (38-39 w.g.). Assessment of surface molecule expression on JEG-3 trophoblast cells was performed using flow cytometer FACS Canto II (BD). JEG-3 cells increased expression of CD116, CD118, CD119, CD181, CD183, CD186, CD192, CCR1, VEGFR1 and CD140a in the presence of first trimester placental supernatants. In the presence of placental supernatants of the third trimester expression of CD116, CD118, CD119, IFNg-R2, CD120b, CD181, CD183, CD186, CD192, CD295, CCR1, EGFR, TGFb-R2 on JEG-3 cells was higher than constitutive expression. Expression of CD116, CD118, CD119, IFNg-R2, CD120b, CD183, CD192, CD295, EGFR and TGFb-R2 on JEG-3 cells was higher after incubation with placental supernatants of the third trimester than after incubation with placental supernatants of the first trimester. Thus, the expression profile of receptors for cytokines, chemokines and growth factors in the presence of placental supernatants from placentas of the first and third trimesters of pregnancy mainly coincided. However, because of receptors increased expression by JEG-3 cells their sensitivity to leptin, cytokines GM-CSF, LIF, IFNg, TNFa, chemokines IP-10, MCP-1, growth factors EGF, TGFb can be higher in the third trimester than in the first trimester.