Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Angela Bentivegna
Posters: J Cell Sci Ther
Transitional cell carcinoma (TCC) comprises the majority of bladder cancers (more than 90%). TCC is grouped in high- or low- grade (HG or LG), noninvasive (NI) or invasive (IN) lesions. Th e biological diff erence between these groups probably refl ects underlying genetic heterogeneity leading to specifi c pathways of tumor development and progression. 22 TCCs (9 HGIN, 3 HGNI, 1 LGIN, 9LGNI) were tested by UroVysion® Bladder Cancer Kit, a fl uorescence in situ hybridization (FISH) assay designed to detect aneuploidy for chromosomes 3, 7, 17 and loss of the 9p21 locus; the test was performed in duplicate on Formalin Fixed Paraffi n Embedded (FFPE) samples and on freshly isolated nuclei (FIN) in order to evaluate the performance of this targeted test in two diff erent materials from the same tumor. At least 100 cells were scored and the signals were divided in loss, disomy and gain (number of signals/cell < 2; = 2; ≥ 3). Statistical analysis was conducted by means of a poisson model. Th e data generated from FIN and from FFPE were generally comparable. However in HGNI signifi cant diff erence was evidenced, except for chromosome 3; conversely in HGIN signifi cant diff erence emerged only for this signal. A second level of comparison was applied on 10 TCC (6 HGIN, 1 HGNI, 3 LGNI) between data derived from array comparative genomic hybridization (array-CGH) and UroVysion analysis on diff erent areas of matched FFPE tissues. Our results refl ect the high intra-tumor heterogeneity and also showed some shared aberrations that could be interesting for the therapy with monoclonal antibodies.
Graduated in Biological Science in 1999, she has a Specialization in Medical Genetics in 2004 and a PhD in experimental Pathology and Neuropathology in 2007. She is scienti fi c researcher c/o Department of Neurosciences and Biomedical Technologies; Assistant Professor of Genetic Pathology, Genetic Markers and Genetic of Riproduction c/o University of Milano Bicocca. Her main interests are pathogenetic mechanisms of genetic syndromic diseases associated with mental retardation and/or congenital heart defects. From 2005 she focused her attention on Mesenchymal Stem Cells (MSCs) and Cancer Stem Cells from Bladder Cancer and Glioblastoma Multiforme. She has published 15 papers, especially the last two concerning the isolation and characterization of the putative bladder cancer stem cell are very cited. She is also a referee for the evaluation of projects for the Italian Ministry of Research and University