Journal of Chromatography & Separation Techniques
Open Access

Microfluidic platforms enabling single-cell electrical property characterization and cell type classification

2^nd International Conference and Expo on Separation Techniques

September 26-28, 2016 Valencia, Spain

Jian Chen

Chinese Academy of Sciences, China

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Size-independent intrinsic electrical properties of single cells i.e., specific membrane capacitance (Cspecific membrane) and cytoplasm conductivity (�?cytoplasm) are promising biophysical markers for cell status evaluation and cell type classification. In this talk, I will present our recent work on developing microfluidic platforms enabling the characterization of Cspecific membrane and �?cytoplasm in a continuous manner. From the perspective of technology development, I will also present four-generation microfluidic platforms for cellular electrical property quantification and highlight the proposed equivalent circuit models for raw electrical data translation. As to applications, I will report the classification of: Mouse tumor samples; Tumor cell lines with single oncogenes under regulation; Paired high and low-metastatic carcinoma strains; and stem cells in the process of differentiation. The microfluidic platforms developed in this study showed cell type classification, which may function as effective tools in further applications.

Biography :

Email: chenjian@mail.ie.ac.cn