Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Sang Hee Shim, Changyeol Lee, Ki-Sung Kang2 and Ji Hoon Song
Duksung Women�s University, South Korea
Gachon University, South Korea
University of Ulsan, South Korea
Posters & Accepted Abstracts: J Chromatogr Sep Tech
Colletotrichum sp. JS-0367 was isolated from Morus alba (mulberry), identified, and cultured on a large scale for chemical investigation. One new anthraquinone (1) and three known anthraquinones (2-4) were isolated and identified using spectroscopic methods including 1D/2D-NMR and HR-ESI-MS. Although neuroprotective effects of some anthraquinones have been reported, the biological activities of the four anthraquinones isolated in this study have not been reported. Therefore, the neuroprotective effects of these compounds were determined against murine hippocampal HT22 cell death induced by glutamate. Compound 4, evariquinone, showed strong protective effects against HT22 cell death induced by glutamate via inhibition of intracellular reactive oxygen species (ROS) accumulation and Ca2+ influx triggered by glutamate. Immunoblot analysis revealed that compound 4 reduced phosphorylation of MAPKs (JNK, ERK1/2, and p38) induced by glutamate. Furthermore, compound 4 strongly attenuated glutamate-mediated apoptotic cell death.
Recent Publications:
1. Pickhardt M, Gazova Z, von Bergen M, Khlistunova I, Wang Y, et al. (2005) Anthraquinones inhibit Tau aggregation and dissolve Alzheimer's paired helical filaments in vitro and in cells. Journal of Biological Chemistry 280:3628�3635.
2. Bringmann G, Lang G, Steffens S, Gunther E, Schaumann K (2003) Evariquinone, isoemericellin, and stromemycin from a sponge derived strain of the fungus Emericella variecolor. Phytochemistry 63:437�443.
3. Nakao N and Brundin P (1998) Neurodegeneration and glutamate induced oxidative stress, Progress in Brain Research 116:245�263.
4. Tan S, Wood M and Maher P (1998) Oxidative stress induces a form of programmed cell death with characteristics of both apoptosis and necrosis in neuronal cells. Journal of Neurochemistry 71:95�105.
5. Gonzalez A G, Barrera J B, Davila B B, Valencia E and Dominguez X A (1992) Anthraquinones from Cassia greggii. Phytochemistry 31:255�258.
Sang Hee Shim has her expertise in Natural Products Chemistry. She has conducted studies on bioactive secondary metabolites from medicinal plants and their associated microbiomes including endophytes. She has also investigated a lot of bioactive secondary metabolites from halophyte-derived microbial cultures and is trying to elucidate interactions between plants and their endophytes.
E-mail: sangheeshim@duksung.ac.kr