Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Neveen Saleh
National Organization for Drug Control and Research, Egypt
Posters & Accepted Abstracts: J Proteomics Bioinform
Acinetobacter baumannii infections are becoming serious health problems with rapid emerging of multidrug strains and has made it difficult to cure, therefore, the development of efficacious treatment strategies against this pathogen is direly needed. One of his strategies could be used are outer membrane proteins (OMPs) of pathogenic bacteria, that have been used as protective antigens in developing bacterial vaccines. This study aimed at trigger immunoprotective effect of outer membrane protein with iron regulated outer membrane protein IROMP vaccination to combat A. baumannii infection. IROMP were extracted using sarkosyl under iron deficient conditions of A. baumannii strains. Bands corresponding to IROMPs were excised from SDS-PAGE and used to immunize mice that efficiently elicited high titers of specific IgG in mice. IROMP immunization suppressed the bacterial burdens in the organs and peripheral blood, and reduced the levels of serum inflammatory cytokines and provided 50% protection against a standardized lethal dose (108 CFU) of A. baumannii in murine pneumonia model. In conclusion IROMP can be used for bactericidal activity against carbapenem resistant A. baumannii.