Immunotherapy: Open Access
Open Access
ISSN: 2471-9552
ISSN: 2471-9552
Zarina Arif
Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, Aligarh U.P., India
Posters & Accepted Abstracts: Immunotherapy (Los Angel)
Statement of purpose: Extensive research till date suggests that oxidative stress, chronic inflammation, and cancer are closely linked. Inflammation results in the production of numerous reactive oxygen, nitrogen and chlorine species, in addition to the products of lipid and sugar oxidation, some of these products are capable of chemically modifying amino acids in proteins. This in turn results in changes to the structure and function of proteins. Increasing evidence demonstrates that such posttranslational modifications result in the generation of neo-epitopes capable of eliciting both innate and adaptive immune responses. Therefore, this study has analyzed the changes in human serum albumin upon modification with peroxynitrite, an endogenously produced powerful oxidizing and nitrating agent, and is implication in the etiopathogenesis of cancer. Methodology & Theoretical Orientation: Various physico-chemical studies were carried out to establish the modification of albumin. Peroxynitrite-modified-albumin exhibited hyperchromicity at 278 nm, changed microenvironment of tyrosine, tryptophan along with the generation of 3-nitrotyrosine, nitrotryptophan and protein carbonyls.
Email: zarif66@hotmail.com