Journal of Glycobiology
Open Access

Proteoglycans extracted from shark cartilage (Echinorhinus brucus) inhibits proliferation of MCF- 7 human breast cancer cells by inducing apoptosis

Glycobiology World Congress

August 10-12, 2015 Philadelphia, USA

Ajeesh Kumar K K

Posters-Accepted Abstracts: J Glycobiol

Abstract :

Proteoglycans were extracted from deep sea shark Echinorhinus brucus cartilage and their anti-proliferative activity against
MCF-7 cell lines was evaluated. Cytotoxicity effect was determined by assessing the cytotoxic effect of the proteoglycans
by MTT assay (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide). The following assays were carried out to find
out apoptosis mechanism was the cause for cytotoxicity, the concentration of caspases 3 and 9, double fluorescent staining
with acridine orange/ethedium bromide and DNA laddering assay. Gene expression assay for BAX, BCL-2 and p53 has been
done to find out the expression of mentioned genes products to confirm apoptosis pathway. Different concentrations such as
5, 10, 25, 50 and 100 �¼g/ml were used for assessing anti-proliferative effect. Significant cytotoxic effect of 75% cell death was
observed for 100 �¼g/ml treated sample. Significant raise in activity of caspase 3 and 9 was found for 100 �¼g/ml treated sample
i.e., 3.92 and 4.35 (expression fold compared with control as 1) respectively. DNA laddering effect was also observed for the
100 �¼g/ml treated sample. Maximum cell death determined by double fluorescent staining was observed for 100 �¼g/ml treated
sample. A significant raise in the fold expression was observed for BCL-2 and p53 of 4.5 and 3.55 respectively (band intensity
was expressed as relative absorbance unit); whereas, low BAX expression of 0.42 was noticed for 100 �¼g/ml treated sample.
Significant anti-proliferative effect was observed for optimal concentration of 100 �¼g/ml treated sample and mechanism behind
cell death was found to be apoptosis as inferred from the above results.

Biography :

Ajeesh Kumar K K has completed his Post graduation in Biochemistry at the age of 22 years from Bharathiar University affiliated college. He has more than three years
experience in biochemistry research field and mainly working on proteoglycans from shark cartilage. He is currently working as a Senior Research Fellow in Central Institute
of Fisheries Technology, Cochin in India and has one national journal publication. He attended two national and international seminars and presented poster and paper.