Translational Medicine
Open Access
ISSN: 2161-1025
ISSN: 2161-1025
Shuxia Li
Posters: Transl Med
Objective: To investigate the hepatic ischemia -reperfusion injury in rats. Methods: According to the reperfusion time, the rats were divided into four groups, sham, ischemia -reperfusion 2 h , 4 h , 24 h group. The blood vessels supplying the median and left lobe were occluded by micro-vascular clamp to achieve 70% liver ischemia for 1 h, the change of liver biochemical markers and pathology were assessed. Results: The survival rate was 100%. Compared with the control group, the hepatic 70% ischemia-reperfusion resulted in severe liver injury with marked elevation in serum aspartate aminotransferase , alanine aminotransferase , tissue malondialdehyde and histological scores (P<0.05). And after 4 h reperfusion, the insult was maximal. Conclusion: Reproducible model of hepatic 70% ischemia -reperfusion injury can be induced by incarcerating the blood vessels of the median and left lobe in rats, and it is recommended to make the optimal hepatic ischemia-reperfusion injury with 4 h reperfusion following ischemia for 1 h. Therefore, this model is useful for the research of hepatic ischemia -reperfusion injury in clinical, which is simple at technical.
Shuxia Li is a Pharmacist in Dept. of Pharmacy, the First Affiliated Hospital, Sun Yat-sen University,China. She completed her medical education in China in 1991 and obtained her MD in 1997 from the University of Tuebinger, Germany. Her research has focused on drug metabolism.