Journal of Developing Drugs
Open Access
ISSN: 2329-6631
+44 1478 350008
ISSN: 2329-6631
+44 1478 350008
Mustafa Abdullah YILMAZ, Abdulselam Ertas, Mehmet Boga and Hamdi TEMEL
Dicle ��niversitesi Science and Technology Application and Research Center, Diyarbak�±r, TURKEY
Dicle University, Faculty of Pharmacy, Department of Pharmacognosy, Diyarbak�±r, TURKEY
Dicle University, Faculty of Pharmacy, Department
Scientific Tracks Abstracts: J Develop Drugs
In this study, major secondary metabolites of 14 different Achillea species that belong to Asteracae family were determined by LC-MS/MS and LC-MS IT-TOF instruments. Also antioxidant and and anticancer properties of these species were investigated. The studied species were taxonomically identified, dried and their overground and underground parts were seperated. The secondary metabolic profiles of the chloroform-methanol (1:1) extracts of the species were determined qualitatively by UHPLC-ESI-IT-TOF MS instrument and 89 metabolites were identified in studied Achillea species. By developing and validating a comprehensive LC-MS/MS method, 37 phytochemicals of chloroform-methanol (1:1) and ethanol extracts of the species were analysed qualitativeley and quantitatively. Additionally, the antioxidant activities of the studied species were determined by using DPPH free radical scavenging assay, ABTS radical cation decolorization assay, �²-carotene lipit peroxidation test system and CUPRAC copper reduction capacity methods. Moreover, total phenolic content and total flavonoid content of the studied species were investigated. Most importantly, the cytotoxic (anticancer) activities (on HeLa (Human Cervical Carcinoma Cell Line) of the chloroform-methanol extracts of the overground and underground parts of the 14 Achillea species were determined.
Mustafa Abdullah Yilmaz is Assistant Professor at Dicle University, Faculty of Pharmacy, Diyarbak�±r, Turkey. He is also in charge of ther mass spectrometry and chromatography unit in Dicle University Science and Technology Application and Research Center (DUBTAM). His research areas are (high resolution) mass spectrometry and chromatography (LC-MS/MS, GC-MS, LC-MS IT-TOF), plant metabolomics, analytical method validation etc. He has published 10 papers in reputed journals.