Advancements in Genetic Engineering
Open Access
ISSN: 2169-0111
+44 1478 350008
ISSN: 2169-0111
+44 1478 350008
Fahad Al-Qurainy, Mohammad Nadeem and Salim Kham
Accepted Abstracts: Adv Genet Eng
Ochradenus arabicus is an important endemic medicinal plant which is found in Saudi Arabia. In vitro culture and propagation protocol was established from seeds of O. arabicus. Synthetic seeds were produced from stem segments growing in vitro . The stem segments were encapsulated in 3.0% sodium alginate prepared in MS medium and 50 mM calcium chloride. Encapsulated beads of stem segments were kept at 4.0C for 8 weeks. The regeneration capacity of synthetic seeds and non encapsulated stem segments were evaluated at 2 weeks interval on MS medium containing 1.0 �¼M BAP. The conversion frequency was high even upto 8 weeks of storage at 4.0C. Rooting in plantlets was obtained on MS medium containing 0.5 �¼M. Thus obtained from synthetic seeds were acclimatized and hardened under greenhouse conditions. The rooted plants established ex vitro and phenotypically all plants were similar to each others as well as with mother plants. Genetically, the plants raised from synthetic seeds and mother plant resulted in monomorphic profile by ISSR marker. This is an efficient and reliable method of synthetic seeds produced and stored for short period (8 weeks at 4.0 C). The method can be useful in raising genetically identical plants from synthetic seeds of O. arabicus. Keywords: Phytohormone; Artificial Seeds; Molecular Markers; Genetic Fidelity