Journal of Infectious Diseases & Preventive Medicine
Open Access
ISSN: 2329-8731
+44 1300 500008
ISSN: 2329-8731
+44 1300 500008
Vilma G Duschak, Luciana L Soprano and Alicia S Couto
National Institute of Parasitol, Argentina University of Buenos Aires, Argentina
Posters & Accepted Abstracts: J Infect Dis Prev Med
Statement of the Problem: Chagas Disease (ChD) constitutes a major endemic health problem in Latin America. The presence of sulfate-bearing-glycoproteins has been identified in trypanosoma cruzi, they are targets of specific immune responses and subjects chronically infected with T. Cruzi mount specific humoral immune responses to sulfated glycoproteins. Cruzipain (Cz), a major antigen. Containing a C-terminal domain (C-T) is responsible for the immunogenicity of the molecule in natural and experimental infection synthetic anionic sugar conjugates containing N-acetyl D glucosamine-6-sulfate (NAcGlc6-SO3) mimics the N-glycan-linked sulfated epitope (sulfotope) displayed in the C-T. IgG2 antibody levels specific for sulfotopes are inversely correlated with chagas disease severity. Another sulfated glycoprotein with serine carboxypeptidase (SCP) activity was studied. Methodology & Theoretical Orientation: Native SCP co-purifies with Cz from Concanavalin-a affinity columns. The Cz-SCP mixture was desulfated, ascribing the cross-reactivity between both molecules to the presence of sulfated groups. SCP-N-glycosydic chains were analyzed by UV-MALDI-TOF-MS. Immunoblotting of lysates from the different parasite stages were confronted with SO3-specific antibodies; in vivo effects of sodium chlorate on Czsulfation and tissue damage in C-T-immunized-mice muscle-tissues were evaluated. Findings: I) The presence of short-sulfated high-mannose-type oligosaccharidic chains was confirmed in SCP II) Sulfotopes participate in trypomastigotes infection of cardiac cells iii) Sulfotopes generate muscle tissue damage in BALB/c mice, in absence of infection iv) sulfotopes from Cz and other Sulfated glycoproteins participate in parasite infection and immunopathogenesis v) Sulfotopes and their specific antibodies are responsible for the ultra-structural abnormalities observed in the outcome of the experimental ChD disease vi) A band with apparent molecular weight similar to SCP was highly recognized in trypomastigotes vi) SCP is a minor antigen recognized by most of chronic-Chagas-disease-patient´s sera. Conclusion & Significance: The shared sulfotopes between Cz and SCP, and the enhanced presence of sulfotopes in trypomastigotes are involved in parasite-host relationship in immunopathogenic and infection processes.
Vilma G Duschak, Doctor in Biochemistry (1989) UBA. CONICET Researcher, Argentina since 1994. Post-grade in Medicine Chile University (1990); Cooperation: Instituto-Cs-Biomedicas- San Pablo-University-Brasil (2005) Universite-Jules Verne-Amiens- France (2007) Bernhard Notch Institute of Tropical Medicine, Hamburg, Germany (2010-2011). Editorial Advisory Board Member, Bentham Science Publishers, USA. Awards and distinctions: 6 Publications: more than 40 Assistance to more than 100 National and international congresses. Directed Thesis: 5 Roche Diagnostics International Meeting experts, New York, USA (2016). Evaluator of research projects from ANPCyT, CONICET and UBA (Argentina), OTKA (Hungary) and European Union international projects, Brussels (2018).