Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Seyed Jamaleddin Shahtaheri,Heravizadeh O R, Khadem M and Nabizadeh R
Tehran University of Medical Sciences,Tehran, Iran
Scientific Tracks Abstracts: J Chromatogr Sep Tech
Background & Aim: Increasing production and the use of pesticides for
agricultural purposes can lead to many environmental and human health risks.
Therefore, trace residue analysis and the assessment of their concentration
in occupational and environmental samples is highly necessary to estimate
human exposure levels. This study was aimed to use a synthesized molecular
imprinted polymer (MIP) as a novel and specific absorbent for residue
analysis of metribuzin herbicide in biological sample.
Method: The MIP was synthesized by copolymerizing the named pesticide
as the template molecule in presence of methacrylic acid and ethylene glycol
dimethacrylate as functional and crosslinking monomers, respectively.
Afterwards, important parameters affecting the adsorption and recycling
process of metribuzin were optimized using synthesized absorbent through
the solid phase extraction (SPE) method to demonstrate the ability of the
synthesized MIP for selective recognition of analyte of interest from
biological sample. All samples were then analyzed by High Performance
Liquid Chromatography.
Results: According to the obtained results, trace amount of herbicide of
interest was successfully determined using synthesized MIP. The optimum
amounts of effective parameters on extraction yield were determined
as follow: adsorbent amount: 13.15 mg, sample pH: 3, sample flow rate:
1 mL/min, volume of extraction solvent: 1.33 mL, flow rate of extraction
solvent: 3 ml/min, and the amount of acid in the solvent composition: 10%.
Detection and quantification limits of the method were 0.0119 and 0.0397
ppb, respectively.
Conclusion: The developed method can be used as an optimum sample
preparation procedure for selective and sensitive extraction procedure and
determination of desired herbicide from biological sample
Seyed Jamaleddin Shahtaheri has completed his PhD from Surrey University, Guildford, Surrey, England in 1996. He is an Academic Member of Department of Occupational Health Engineering, Tehran University of Medical Sciences, Iran; acting as the Dean Research Deputy at Institute for Environmental Research in the same University. He served as Persistent Organic Pollutant Review Committee (POPRC) Member under the Stockholm Convention, UNEP, UN during the period 2014-2018
E-mail: shahtaheri@tums.ac.ir